I've got 30 hours on my machine now. Honestly, I don't know if I would be able to tell if it loosened up or not. Over the course of a year or two, after you've accumulated 100 hours, I wouldn't know whether I simply got used to it or it got looser.
If you really wanted to know, you could take some sort of scale and try to measure the lbs to move the pedal. And then remeasure in the same fashion after 50 hours and see if there is any change.
For me, after owning mine since June of 2020 and putting 30 hours on mine, I've basically gotten used to it. If there was a simple fix I'd think about it, but other than that it is what it is. The B2601 is a fantastic machine in terms of features, size and capability. The big shortcomings are the seat suspension/base (the seat itself is fine) and the pedal stiffness.